灯盏乙素抗新生大鼠缺氧缺血性脑损伤的作用及对 p38 MAPK 表达的影响

医学研究与教育 ›› 2015, Vol. 32 ›› Issue (2): 6-10.

灯盏乙素抗新生大鼠缺氧缺血性脑损伤的作用及对 p38 MAPK 表达的影响

王琳¹，牛培²，武变瑛²

1. 河北大学中医学院，河北保定 071000；2. 河北大学基础医学院，河北保定 071000

出版日期:2015-04-25 发布日期:2015-04-25
通讯作者: 武变瑛（1958—），女，河北保定人，教授，硕士生导师，主要从事器官缺血再灌注损伤及脑记忆功能的研究。E-mail: wuby9026@126.com
作者简介:王琳（1987—），女，河北保定人，在读硕士。E-mail: wanglin1110@126.com
基金资助:
河北省中医药管理局计划项目（2011032）；河北省人口计生委计划项目（2012-A02）

Anti-injury effect of scutellarin on the hypoxic ischemic brain damage of neonatal rats and the expression of p38 MAPK

WANG Lin¹, NIU Pei², WU Bianying²

1. College of Traditional Chinese Medicine of Hebei University, Baoding 071000, China; 2. College of Basic Medical Science of Hebei University, Baoding 071000, China

Online:2015-04-25 Published:2015-04-25

摘要/Abstract

摘要： 目的探讨灯盏乙素抗新生大鼠缺氧缺血性脑损伤的作用及对p38 MAPK表达的影响及机制。方法 45只健康新生 7 日龄 SD 大鼠随机分为假手术组、缺血缺氧组、灯盏乙素组（25 mg/kg）。分别于用药后第 24 小时、3 天、7 天每组处死大鼠各 5 只，取脑组织切片。采用 TUNEL染色检测神经细胞凋亡；采用免疫组织化学染色检测 p38 MAPK 的表达高峰、表达变化趋势。结果缺氧缺血后海马 CA1 区神经细胞凋亡，p38 MAPK 表达增加；灯盏乙素组神经细胞凋亡及p38 MAPK 表达均减少（与同一时间点缺氧缺血组比较差异有统计学意义 P<0.05）。结论灯盏乙素可能是通过抑制 p38 MAPK 信号通路而减少神经细胞凋亡，从而发挥脑保护作用。

关键词: 灯盏乙素, 缺氧缺血, 脑, p38 MAPK, 凋亡

Abstract: Objective To study the effects of scutellarin treat HIBD in neonatal rats and the expression of p38 MAPK signal pathway following HIBD and its mechanism. Methods 45 healthy 7-day-old neonatal SD rats were randomly divided into sham operation group, HIBD group, and scutellarin group(25 mg/kg). After medication, each group respectively executed 5 rats in 24 h, 3 d, 7 d, and took brain tissue slice . The neuronal apoptosis was detected using TUNEL assay. The highest expression and expressional tendencies of p38 MAPK was determined by the method of immunohistochemistry. Results The apoptotic neurons, actived p38 MAPK increased after HIBD in the hippocampal CA1 region. The expression level of p38 MAPK and apopotic neurons in scultellarin group were significantly lower than HIBD group (the differences of the same time point were statistically significant P<0.05 ). Conclusion Scultellarin could reduce the apoptosis through inhibiting the p38 MAPK signal pathway with cerebral hemorrhage.

Key words: scutellarin, hypoxia-ischemia, brain, p38 MAPK, apoptosis

中图分类号:

王琳，牛培，武变瑛. 灯盏乙素抗新生大鼠缺氧缺血性脑损伤的作用及对 p38 MAPK 表达的影响[J]. 医学研究与教育, 2015, 32(2): 6-10.

WANG Lin, NIU Pei, WU Bianying. Anti-injury effect of scutellarin on the hypoxic ischemic brain damage of neonatal rats and the expression of p38 MAPK[J]. Medical Reserch and Education, 2015, 32(2): 6-10.

参考文献

[1] ARANGO J I, ALLRED K, ADELSON P D, et al. Hypothermia in hypoxic ischemic encephalopathy: A 5-year Experience at Phoenix Children’s Hospital Neuro NICU[J]. Adv Pediatr, 2014, 61(1): 215-223.
[2] GUO Hong, HU Limin, WANG Shaoxia, et al. Neuroprotective effects of scutellarin against hypoxic-ischemic-induced cerebral injury via augmentation of antioxidant defense capacity[J]. Chin J Physiol, 2011, 54(6): 399-405.
[3] HU Xiamin, ZHOU Mimei, HU Xianmin, et al. Neuroprotective effects of scutellarin on rat neuronal damage induced by cerebral ischemia/reperfusion[J]. Acta Pharmacol Sin, 2005, 26(12): 1454-1459.
[4] 欧阳福连, 周细中, 方素珍, 等. 新生大鼠缺氧缺血性脑损伤远期行为学和超微结构改变[J]. 中国当代儿科杂志, 2012, 14(5): 380-384.
[5] RADULOVA P, SLANCHEVA B. Neonatal hypoxic-ischemic brain injury: pathogenesis and neuropathology[J]. Akuah Ginekol(Sofoila), 2014, 53(3): 41-47.
[6] BLOMGREN K, LEIST M, GROC L. Pathological apoptosis in the developing brain[J]. Apoptosis: an international journal on programmed cell death, 2007, 12(5): 993-1010.
[7] CUADRADO A, NEBREDA A R. Mechanisms and function of p38 MAPK signalling[J]. Biochem J, 2010, 429(3): 403-417.
[8] THORNTON T M, PEDRAZA-ALVA G, DENG B, et al. Phosphorylation by p38 MAPK as an alternative pathway for GSK3beta inactivation[J]. Science, 2008, 320(5876): 667-670.
[9] LEE M S, CHAO J, YEN J C, et al. Schizandrin protects primary rat cortical cell cultures from glutamate-induced apoptosis by inhibiting activation of the MAPK family and the mitochondria dependent pathway[J]. Molecules, 2012, 18(1): 354-372.
[10] CARDACI S, FILOMENI G, ROTILIO G, et al. p38(MAPK)/p53 signalling axis mediates neuronal apoptosis in response to tetrahydrobiopterin-induced oxidative stress and glucose uptake inhibition: implication for neurodegeneration[J]. Biochem J, 2010, 430(3): 439-451.
[11] CHU Qingcui, WU Ting, FU Liang, et al. Simultaneous determination of active ingredients in Erigeron breviscapus (Vant.) Hand-Mazz by capillary electrophoresis with electronchemical detection[J]. J Pharm Biomed Anal, 2005, 37(3): 535-541.
[12] 李丽, 刘东阳, 江骥, 等. 灯盏乙素药理学研究进展[J]. 中草药, 2006, 37(8): 附 9-附 11.
[13] 张明艳, 范淑娟, 李利平, 等. 灯盏花注射液抗新生鼠缺氧缺血性脑损伤的作用及对 Bcl-2、Bax 蛋白表达的影响[J]. 中国应用生理学杂志, 2011, 27(2): 196-200.

[1]	卢丹丹,王佩,李蒙蒙,万莉,李轩,赵婧. 核磁负担评分与轻型脑卒中早期神经功能恶化的相关性[J]. 医学研究与教育, 2023, 40(4): 19-24.
[2]	黄雨青,王艳萌,杨信才. 脑卒中偏瘫患者步态训练的现状分析[J]. 医学研究与教育, 2023, 40(3): 46-54.
[3]	房翠,王秀丽,李玲钰,史福平. 缺血性脑卒中后抑郁的发病机制及新生物学指标[J]. 医学研究与教育, 2023, 40(2): 18-24.
[4]	王雪静,谢仙萍,吴志萍,谢婷. 中西医结合护理在脑卒中吞咽障碍康复中的研究进展[J]. 医学研究与教育, 2023, 40(2): 66-73.
[5]	王艳萌,王宁,隗麒轩,黄雨青,杨信才. 以三甲医院为主导的康复管理体系对脑卒中偏瘫患者的影响[J]. 医学研究与教育, 2023, 40(1): 29-32.
[6]	黄显义, 刘霜, 刘艳, 岳洁, 陈丽. 脑卒中患者恐惧疾病进展的研究现状[J]. 医学研究与教育, 2022, 39(5): 68-74.
[7]	谢瑞娟,邓赟,刘涓,杨楠,张堃. 以感觉刺激为导向的易化技术对脑卒中后上肢功能康复效果的影响[J]. 医学研究与教育, 2022, 39(4): 14-21.
[8]	朱健平,李运曼. Nrf2在缺血性脑损伤中的关键性作用[J]. 医学研究与教育, 2022, 39(2): 1-9.
[9]	杨阳,王珏,刘宁. 急性脑卒中患者吞咽障碍检出率的Meta分析[J]. 医学研究与教育, 2022, 39(2): 54-65.
[10]	隋莲玉,殷小平,王佳宁. 肺癌脑转移MRI影像基因组学研究进展[J]. 医学研究与教育, 2022, 39(1): 25-34.
[11]	张孟繁,王秀丽,路潇,张文会,梁璐. 肠道菌群与缺血性脑卒中相关研究进展[J]. 医学研究与教育, 2021, 38(6): 8-14.
[12]	李雪霏,姜媛媛,田帆,井坤娟,曹畅. 脑卒中患者病耻感现状及影响因素研究[J]. 医学研究与教育, 2021, 38(6): 59-68.
[13]	庄建港. 替罗非班对急性脑梗死机械取栓患者氧化应激水平及神经功能的影响[J]. 医学研究与教育, 2021, 38(5): 20-25.
[14]	瞿海龙,张红强,张冰,梁璐. 脑肺超声联合实现脑保护通气策略个体化[J]. 医学研究与教育, 2021, 38(4): 23-28.
[15]	侯岚,梁超,张舒寒,王佩,贾同乐. 无高血压及糖尿病者脑微出血危险因素[J]. 医学研究与教育, 2021, 38(3): 7-11.