乳腺癌中SLC5A8基因的甲基化及转录水平

doi:10.3969/j.issn.1674-490X.2020.01.007

医学研究与教育 ›› 2020, Vol. 37 ›› Issue (1): 36-40.DOI: 10.3969/j.issn.1674-490X.2020.01.007

乳腺癌中SLC5A8基因的甲基化及转录水平

刘岩^1,2, 刘雅涵³, 谢天皓³, 孟庆旭³, 靳小石^2,3

1.河北大学临床医学院, 河北保定 071000;
2.河北省普通外科数字医学基础研究重点实验室, 河北保定 071000;
3.河北大学附属医院, 河北保定 071000

收稿日期:2020-01-08 出版日期:2020-02-25 发布日期:2020-02-25
通讯作者: 靳小石(1973—),男,河北保定人,主任医师,博士,硕士生导师,主要从事胃肠、甲状腺、疝研究。E-mail: doctorjinxiaoshi@126.com
作者简介:刘岩(1991—),男,河北邢台人,在读硕士,主要从事普外科研究。 E-mail: doctorliuyan1221@163.com
基金资助:
保定市科学技术研究与发展计划项目(17ZF238)

Received:2020-01-08 Online:2020-02-25 Published:2020-02-25

摘要/Abstract

摘要： 目的研究SLC5A8基因在乳腺癌中的甲基化及转录水平。方法将2017年5月至2019年12月河北大学附属医院基本外科的32例乳腺癌患者经手术治疗的肿瘤标本及癌旁正常组织标本分为2组,分别为实验组和对照组。采用甲基化特异性PCR法检测32例乳腺癌及乳腺癌周围正常组织SLC5A8基因甲基化,运用RT-PCR法检测32例乳腺癌组织SLC5A8基因的转录表达水平情况。结果实验组32例乳腺癌组织SLC5A8基因甲基化阳性20例(62.50%),对照组32例癌旁组织SLC5A8基因甲基化阳性7例(21.87%)。实验组的甲基化阳性率显著高于对照组(P<0.05)。实验组甲基化阳性的20例乳腺癌组织中检测到基因mRNA转录表达的有6例(30.00%)。实验组甲基化阴性的12例癌组织中检测到基因mRNA转录表达的有7例(58.33%)。实验组中甲基化阳性的癌组织基因转录表达率较甲基化阴性的癌组织基因转录表达率显著降低(P<0.05)。结论 SLC5A8基因甲基化是乳腺癌中普遍存在的一种现象,可能参与乳腺癌的演变过程,并且乳腺癌组织SLC5A8基因甲基化与基因的转录表达不是完全一致的,二者具有一定的反向一致性。

关键词: SLC5A8基因, 乳腺癌, 甲基化

Abstract: Objective To explore the methylation and transcription level of SLC5A8 gene in breast cancer. Methods The tumor samples and adjacent normal tissue samples of 32 cases of breast cancer patients treated with basic surgery in Affiliated Hospital of Hebei University from May 2017 to December 2019 were divided into two groups, namely the experimental group and the control group. Methylation of SLC5A8 gene in 32 cases of breast cancer and surrounding normal tissues was detected with methylation-specific PCR, and transcriptional expression of SLC5A8 gene in 32 cases of breast cancer was detected with RT-PCR. Results There were 20(62.50%)positive cases of SLC5A8 methylation in 32 breast cancer tissues in the experimental group and 7(21.87%)positive cases of SLC5A8 methylation in 32 adjacent tissues in the control group. The methylation positive rate in the experimental group was significantly higher than that in the control group(P<0.05). mRNA transcription was detected in 6 of the 20 breast cancer patients(30.00%). mRNA transcription was detected in 7(58.33%)of the 12 cancer tissues with negative methylation in the experimental group. In the experimental group, the transcription expression rate of methylated positive oncotissue was significantly lower than that of methylated negative oncotissue(P<0.05). Conclusion SLC5A8 gene methylation is a common phenomenon in breast cancer, which may be involved in the occurrence and development of breast cancer, and The methylation of SLC5A8 gene in breast cancer tissues is not completely consistent with the transcriptional expression of the gene, and there is a certain inverse consistency between the two.

Key words: SLC5A8 gene, breast cancer, methylation

中图分类号:

刘岩, 刘雅涵, 谢天皓, 孟庆旭, 靳小石. 乳腺癌中SLC5A8基因的甲基化及转录水平[J]. 医学研究与教育, 2020, 37(1): 36-40.

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乳腺癌中SLC5A8基因的甲基化及转录水平

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