Influence of nanoparticle on the structure of protein studied by atomic force microscopy

doi:10.3969/j.issn.1674-490X.2015.06.001

Abstract

Abstract: Objective To study the effects of different characteristics of nanoparticles on the structure of protein. Mathods Using atomic force microscopy (AFM) to characterize the changes of nanoparticles sizes before and after protein adsorption, and then the effects on the structure of protein were determined. Results In the aspect of the nanoparticles size, the binding of protein onto smaller nanoparticles resulted in smaller change of the protein structure. In the aspect of the materials of nanoparticles, effect of the semiconductor nanoparticles on the structure of protein was greater than that of the gold nanoparticles. In the aspect of the shape of nanomaterial, the nanosheets of graphene oxide (GO) had greater influence on the deformation of protein structure than spherical nanoparticles. Conclusion The particle size, material, and shape have significant effects on the structure of protein.

Key words: protein, nanoparticles, atomic force microscopy

CLC Number:

TB39

GAO Hongyun, YANG Chao, XI Yanan, YANG Tao. Influence of nanoparticle on the structure of protein studied by atomic force microscopy[J]. Medical Reserch and Education, 2015, 32(6): 1-10.

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