Anti-injury effect of scutellarin on the hypoxic ischemic brain damage of neonatal rats and the expression of p38 MAPK

Abstract

Abstract: Objective To study the effects of scutellarin treat HIBD in neonatal rats and the expression of p38 MAPK signal pathway following HIBD and its mechanism. Methods 45 healthy 7-day-old neonatal SD rats were randomly divided into sham operation group, HIBD group, and scutellarin group(25 mg/kg). After medication, each group respectively executed 5 rats in 24 h, 3 d, 7 d, and took brain tissue slice . The neuronal apoptosis was detected using TUNEL assay. The highest expression and expressional tendencies of p38 MAPK was determined by the method of immunohistochemistry. Results The apoptotic neurons, actived p38 MAPK increased after HIBD in the hippocampal CA1 region. The expression level of p38 MAPK and apopotic neurons in scultellarin group were significantly lower than HIBD group (the differences of the same time point were statistically significant P<0.05 ). Conclusion Scultellarin could reduce the apoptosis through inhibiting the p38 MAPK signal pathway with cerebral hemorrhage.

Key words: scutellarin, hypoxia-ischemia, brain, p38 MAPK, apoptosis

CLC Number:

WANG Lin, NIU Pei, WU Bianying. Anti-injury effect of scutellarin on the hypoxic ischemic brain damage of neonatal rats and the expression of p38 MAPK[J]. Medical Reserch and Education, 2015, 32(2): 6-10.

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