doi:10.3969/j.issn.1674-490X.2017.01.007

Abstract

Abstract: Myriads of studies indicate that sperm with severe head morphological abnormalities is closely associated with low fertilization,implantation and pregnancy rate.Selecting high quality sperm used for ART(assisted reproductive techniques)is an effective way to improve the clinic outcome.A normal sperm selected under a optical microscope with low magnification(×200)could contain some ultrastructural defects,which may result in fertilization failure or arrest of embryonic development after ICSI(Intracytoplasmic sperm injection).However,intracytoplasmic morphologically selected sperm injection(IMSI)shows the perfect match between ICSI and MSOME(Motile sperm organelles morphology examination),which can evaluate the quality of sperm morphology and cherry-pick sperm with none or less vacuole for microinjection to increase the rates of fertilization,implantation and pregnancy,as well as decrease the abortion rate.The examination is performed using a differential interference contrast microscopy and digital imaging technologyto achieve a magnification up to 6 600×.This review mainly focus on the development history of IMSI techniques,its application in optimal selection of sperm and analysis of clinical outcomes,which will provide some theoretical directions to its application popularization and further development.

Key words: intracytoplasmic morphologically selected sperm injection(IMSI), motile sperm organelles morphology examination(MSOME), sperm vacuoles, optimal selection of sperm

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References

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